Gram Negative Coccobacilli
A 20-year-old man presents to the clinic with a history of fever and nonproductive cough. The patient's chest x-ray shows consolidation of the right lower lobe. An infection with Mycoplasma pneumoniae is considered as the cause of the patient's pneumoniA. Which of the following methods would confirm this diagnosis?
|A||Culture of sputum specimen on solid medium|
|B||Detection of organism by microscopy|
|C||Complement fixation test of acute and convalescent sera|
|D||PCR amplification of patient's sputum specimen|
Ref-Mandell’s Principles and Practice of infectious diseases,6th ed, pg 2276
a. Culturing M. pneumoniae is difficult and slow and is not used for diagnosis;
b. Mycoplasmas lack a cell wall making microscopy inappropriate;
c. PCR amplification of a sputum specimen is a specific and rapid diagnostic method.
d. Complement fixing antibodies though specific do not arise early enough to be helpful in diagnosis.