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Microbiology

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Gram Negative Bacilli

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A young boy from Himachal Pradesh presented with small ulcer on leg, . After 5 days he developed fever and inguinal lymphadenopathy. A smear was sent to the laboratory to perform a specific bipolar staining. Which one of the following staining method would help in the identification of the suspected pathogen:



A Albert staining

B Zeihl-Neelson staining

C Mc Fadyean’s staining

D Wayson staining

Ans. D Wayson staining

Wayson staining.

(Ref. Textbook of microbiology by Ananathanarayan 6th ed. 224, 299, 360)

Rat flea is an arthropod responsible as a vector for transmission of diseases like:

• Bubonic plague, • Endemic typhus • Leptospirosis.

a. Yersinia pestis show bipolar staining (safety pin appearance) in smears stained with Giemsa or methylene blue / Wayson staining..

b. When blood films containing anthrax bacilli are stained with polychrome methylene blue, an amorphous purplish material is seen around the bacilli, which represents capsular material and is characteristic of anthrax bacillus (Mc Fadyean’s staining).

Endemic or flea-borne or murine typhus is caused by R. mooseri, which stain bluish purple with Giemsa and Castaneda and deep red with Machiavello and Gimenez stains.

Mycobacterium tuberculosis, Mycobacterium leprae, Nocardia, Cyclospora, Cryptosporidium, Isospora and Bacterial spores are stainable By Ziehl-Neelsen staining.

Clinical feature of plague

Gram Negative Bacilli Flashcard List

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