All of the following forces are involved in Antigen antibody reaction except: (AIPG 09)
|A||Vander Waal's forces|
Antigen-antibody reaction involves weak interactions like electrostatic interaction, vander waal forces, hydrogen bonds and hydrophobic interaction. Covalent bond is a strong bond and is not present in antigen-antibody complex.
Affinity is Strength of reaction between a single antigenic determinant and a single antibody combining site.
Avidity is the over all strength of binding between an antigen with many determinant & multivalent antibodies.
1. All antibodies are immunoglobulins (Ig) Ig are glycoproteins consisting of 2 light chain and 2 heavy chain.
2. Heavy chains are structurally and antigenically distinct for each class while L chains are similar (either κ
or λ). Κ = 60%, λ = 40%. * λ changed are of 4 types. λ1 λ2 λ3 λ4
3. Ag combining site is at amino terminus composed of both H & L chains.
4. L chain consists of 1 variable and 1 constant region while H chain has 1 variable and 3 or more constant region. Variable regions are responsible for antigen binding and constant region are responsible for biologic function. Each variable region has hyper variable region or complementarity determining region. The analytical plot for hyper variability region is called Wu-Kabat analysis
5. Fab fragment consists of both H and L chain while Fc fragment has only H chain.
6. IgM and IgE are devoid of hinge.