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Pathology

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Immunopathology

Question
2 out of 11
 

Amyloid deposits stain positively with all of the following except? (AIIMS May 2013)



A Congo red
B Crystal violet

C Methenamine silver
D Thioflavine T

Ans. C i.e. Methamine silver [Ref: Harsh Mohan 5th/e p. 89]

Staining characteristics of amyloid

Stain on Gross

Stain used - Lugol's iodine

Appearance: Imparts purple colour, on addition of dilute sulfuric acid turns blue

Hematoxylin & Eosin

Stain used - Hemotoxylin & Eosin

Appearance - On light microscopy appear as extracellular homogeneous structureless eosinophilic hyaline material

Metachromatic stains (Rosamine dyes)

Stains used - Methyl violet and crystal violet

Appearance - Rose pink coluration of amyloid-deposits

Congo red and polarised light

(All types of amyloid have affinity for congo red stain)

Stain used - Congo red

Appearance - Ordinary light - Pink

Polarised - Apple green birefringence light

Fluorescent stains

Stain used - Thioflavin-T

Appearance - yellow under ultraviolet light

Immunohistochemistry

Stain used - various antibody stains against the specific antigenic protein types e.g. Anti

AP, anti AA, anti Lambda, anti kappa

Non specific stains

Stain used Toluidine blue

Alcian blue

Periodic acid Schiff

Colour Orthochromatic blue

Blue green colour

Immunopathology Flashcard List

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