Cell fusion is an innovative technique of prep-aring specific antibodies. Techniques to induce the cell-fusion include the following except:
|A||By adhering some inactivated viruses to surface membrane|
|B||By adding component polyethylene glycol|
|C||By mild electric shock|
|D||By reducing the viscosity of the membrane|
Cell fusion is an innovative technique of preparing specific antibodies as applied in hybridoma technology.
Techniques to induce the cell-fusion include:
o By adhering some inactivated viruses to surface membrane
o By adding component polyethylene glycol
o By mild electric shock.
a. Antibodies produced by a single clone of plasma cells and directed against a single antigenic determinant are called monoclonal antibodies.
b. Large scale production of monoclonal antibodies for use in diagnostic/ therapeutic/ research purposes was 1st introduced by Kohler and Milstein-Nobel Prize in 1984 .
c. Involve the use of hybridomas produced by fusing antibody producing B cells and myeloma (cancer) cells
d. Myeloma cells have lost the ability to produce antibodies and lack HGPRT enzyme.
e. Mouse spleen plasma cells sensitized to produce ab’s against the desired antigen are isolated and fused with myeloma cells using polyethylene glycol or the Sendai virus or electrofusion which uses high-voltage electric pulses.
f. Hybridoma cells are grown in HAT medium to separate the unfused myeloma cells