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Microbiology

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General Microbiology

Question
83 out of 97
 

DNA can be transferred from one bacterium to another by a number of processes. Uptake by a recipient cell of soluble DNA released from a donor cells is defined as which one of the following?



A Competence
B Conjugation

C Recombination
D Transformation

Ans. D Transformation

a. Acquisition of soluble DNA from environment and incorporation in its genome is called transformation

b. First demonstrated in S. pneumoniae by Griffith 1928

c. DNA must be derived from closely related strain

d. Cells that can take up naked DNA are called as competent

EXTRA EDGE

TRANSPORT MEDIA

Pike's media

VR media, APW

Cary-Blair media

Stuart media

Sach's media, SS media

Streptococcus pyogens

Vibrio

Vibrio cholerae, shigella, salmonella, campylobacter

N. gonococci, bordetella

Shigella

IMP. Media

Anthrax bacillus

PLET medium

Bordetella

Bordet Gengou medium/glycerine potato BA

Brucella

Castaneda medium, Trypticase soy agar and broth

Campylobacter jejuni

Campy BAP, Skirrow or Butzler’s media

Chlamydia

HeLa cells, McCoy

Clostridia (Anaerobic organisms)

Robertson’s cooked meat broth

Corynebacterium diphtheriae

Loeffler’s serum slope, tellurite blood agar

Neisseria gonorrhoeae

Thayer-Martin medium, Chacko nair

NYC medium, Chocolate agar

Pseudomonas

Cetrimide agar

Shigella

Deoxycholate Citrate agar (DCA).HE agar

Spirochetes

Smith Noguchi’s medium

To differentiate lactose and non lactose fermenters

Mac Conkey’s agar, Eosin Methylene Blue (EMB) agar

Pasturella/Yersinia

Ghee broth

Acanthamoeba and Naeglaria

E.coli enrichment on non-nutrient agar

Vibrio

TCBS, Bile salt agar, Monsur’s medium

Salmonella

Wilson-Blair bismuth sulfide, HE agar

+ LJ media

+ Sabouraud's dextrose agar

+ NYC agar

+ NNN media, Hockmeyer’s, Schneider’s

+ BCYE media, Feeley-Gormann’s

+ CNA agar media

+Modified Kelly’s medium

+EMJH, Korthoff’s, Fletcher’s medium

- Mycobacteria

- fungi

- Neisseria gonorrhoeae

- Lieshmania

- Legionella

- for selective isolation of gram + cocci

- Borrelia burgdorferi

- Leptospira

· A special media Mannitol, egg yolk, phenol red, polymyxin; agar (MYPA) medium is used for

isolation of B. cereus from feces and other sources.

q APPEARANCE ON CULTURE MEDIA

Appearance

Microorganism

1)Medusa head, string of pearl colonies, inverted fir tree appearance

2)Daisy head /poached egg colonies

3)Satellitism

4)Checkers colonies

5)Bisected pearl, Mercury drop, Aluminium paint

6)Fried egg colonies

7)Stalactite growth in ghee broth

8)Draughtsman /carrom coin appearance

9)Molar tooth appearance

B. anthracis

C. diphtheria on tellurite media

H. influenza

Pneumococci on blood agar

Bordetella pertussis

Mycoplasma

Y. pestis

Pneumococcus

Actinomyces

General Microbiology Flashcard List

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