Gram Negative Bacilli
Diagnostic test for ElEC is:
|B||HeLa cell invasion|
HeLa cell invasion and Sereny test
a. Diagnostic tests for EIEC includes Sereny test, in which instillation of suspension of EIEC or Shigella into the eyes of guinea pig leads to mucopurulent conjunctivitis and severe keratitis.
b. Another test is cell penetration of HeLa or Hep-2 cells and detection of virulence marker antigens (VMA) by ELISA.