Enzyme + Coenzyme constitutes
a. Each enzyme has its own TERTIARY STRUCTURE and specific conformation, which is very essential for its catalytic activity (= Biological activity).
b. The functional unit of Enzyme is known as Holoenzyme
c. Holoenzyme = Apoenzyme + coenzyme
(Active enzyme) (Protein part) (Non-protein part)
d. The term prosthetic group is used when the non-protein moiety tightly (covalently) binds with apoenzyme.
e. The co-enzyme can be separated by dialysis from the enzyme while the prosthetic group cannot be.
f. Enzymes may be defined as biocatalysts synthesized by living cells. They are protein in nature (exception –
RNA acting as ribozyme), colloidal and thermolabile in character, and specific in their action.