Fluores amine is used to detect
Ninhydrin or Fluores amine detect Amino acids
a. Ninhydrin →oxidatively — decarboxylate α-Amino-acids to Co2; NH3 and Aldehyde → forming blue
b. complex that maximally absorbs light at 570 nm wavelength →detect as little as 1μg Amino acid.
c. Proline and Hydroxyproline — Produces Yellow colour with Ninhydrin
d. Fluores amine — more sensitive → detect even nanogram of Amino acid.