Sterilization and disinfection
Following are least susceptible to disinfectants and antiseptics?(AIPG 2009)
a. Most resistant to disinfectants and antiseptics are prions.
b. Protozoa, spores and fungi can be killed by autoclaving, hot air oven, gamma radiation or chemisterilants like Cidex, ethylene oxide or plasma.
c. Prions are killed by autoclaving for 18 minutes at a temperature of 134 C or 25% sodium hypochlorite. They are resistant to gamma radiation or chemisterilants like Cidex, ethylene oxide or plasma.