A. All bacterial have 3 major nutritional needs
- Source of carbon (cellular components)
- Source of nitrogen (proteins)
- Source of energy (synthesis of macromolecules & to maintain chemical gradients)
Basic building blocks required for growth are the same for all cells; bacteria vary widely in their ability to use different sources of these molecules
E-Test = a diffusion method of sensitivity testing, uses the principle of predefined antibiotic gradient on a plastic strip to generate a MIC value API (manual) , Vitek (automated), Microscan (manual or automated) are commercial bacterial identification systems where as Vitek 2 is automated antimicrobial susceptibility test system in which algorithmic analysis of growth kinetics is performed by systemâ€™s software to derive MIC data, available as early as 6-8 hrs for most clinically relevant bacteria.