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Pathology

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Genetics

Question
3 out of 9
 

In-situ DNA nick end labeling can quantitate - (AIPG 2011)



A Fraction of cells in apoptotic pathways

B Fraction of cells in S phase

C p53 gene produce

D bcr/abl gene

Ans. A Fraction of cells in apoptotic pathways [Ref www.chestjoural.org]

In-situ DNA nick and labeling

a. DNA breaks can be detected by terminal deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate in situ nick end-labeling (TUNEL).

b. Apoptosis is currently one of the most intensively researched areas of science, and numerous ways exist to measure the process. Among the morphological, biochemical and molecular changes occurring in the dying cells, DNA fragmentation is widely recognized as a hallmark of apoptosis, and its detection is the most common analysis for identifying Apoptosis.

c. Cleaved chromosal DNA of apoptosis can be detected by in situ DNA nick end-labeling.

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