Number of bonds broken in protein synthesis is
- Peptide Bond formation (Protein synthesis) : requires hydrolysis of high energy phosphates bonds - as follows
i. The charging of the tRNA molecule with amino acylmoiety require hydrolysis of an A TP to an AMP, equivalent to the hydrolysis of 2 ATPs to 2ADPs and phosphates.
ii. The entry of the aminoacyl-tRNA into A site results in the hydrolysis of one GTP to GDP (3) The translocation of the newly formed peptidyl-tRNA in the A site into the P site by eEF-2 similarly result in the hydrolysis of GTP to GDP and phosphate.
- "Thus, the energy requirements for the formation of one peptide bond include the equivalent of the hydrolysis of 2ATP molecules to ADP and 2 GTP molecules to GDP, or the hydrolysis of 4-high energy phosphate bonds.