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Biochemistry

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Purines, Pyrimidines and Nucleic Acid Metabolism

Question
26 out of 59
 

Presence of which of the following in the expression of vector ensures increase in the yield of recombinant protein produced? (AIPG 2010)



A Inducible promoter

B Gene coding for protease inhibitor

C Translation initiation signals

D Transcription and Translation termination signals

Ans. A Inducible promoter.

a. The primary advantage of an inducible promoter expression system is that production of the recombinant protein can be biochemically controlled, allowing for the separation of unique growth and production phases of the culture.

b. During the growth phase, the culture is rapidly grown to high cell density prior to induction without the extra metabolic burden of exogenous protein production, thus minimizing the nonproductive period of the culture. Induction of the culture at high cell density ensures that the volumetric production will be maximized.

Thus, a Inducible promoter in the expression of vector increases the yield of recombinant protein produced.

Given the importance of protease inhibitors, characterization of protease inhibitors which can play a role in preventing, ameliorating or correcting duysfunctions and diseases.

Purines, Pyrimidines and Nucleic Acid Metabolism Flashcard List

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