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Biochemistry

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Amino Acids, Proteins and Porphyrins

Question
20 out of 36
 

Process in which exchange of ions in solution form with that on to surface form occurs in(AIPG 2010)



A Ion exchange

B Paper chromatography

C SDPG

D Anionic chromatography

Ans. A Ion-exchange

Explanation:

a. Ion-exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their charge.

b. It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids. The solution to be injected is usually called a sample, and the individually separated components are called analytes.

c. It is often used in protein purification, water analysis, and quality control. Ion exchange chromatography retains analyte molecules on the column based on coulombic (ionic) interactions.

d. The stationary phase surface displays ionic functional groups (R-X) that interact with analyte ions of opposite charge. This type of chromatography is further subdivided into cation exchange chromatography and anion exchange chromatography.

e. The ionic compound consisting of the cationic species M+ and the anionic species B- can be retained by the stationary phase. Cation exchange chromatography retains positively charged cations because the stationary phase displays a negatively charged functional group:

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