Speckled pattern is seen in - (AIIMS Nov 2013)
|A||Anti Sm antibody|
|B||Anti ds DNA antibody|
|C||Anti histone antibody|
|D||Anti chromatin antibody|
a. The most commonly used method to detect antinuclear antibodies is indirect immunofluorescence.
b. The pattern of nuclear fluorescence suggests the type of antibody present in patient's serum.
c. Four basic patterns are recognized :-
i. Homogenous or diffuse nuclear chromatin staining → Reflects anti histone Q and anti chromatin antibodies.
ii. Rim or peripheral staining →Anti ds DNA antibody. Q
iii. Speckled pattern → Most common pattern on immunofluorescence. Reflects the presence of non DNA nuclear contents → anti Sm Q, anti - Ro, anti-La, anti SS-A, and anti SS-B antibodies.
iv. Nucleolar pattern → Nucleolar RN