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Biochemistry

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Amino Acids, Proteins and Porphyrins

Question
27 out of 36
 

Structure of proteins can be determined by all except? (AIIMS Nov 2008)



A Mass spectrometry

B NMR spectrometry

C HPLC

D Edman’s degradation technique

Ans. C HPLC

Detection of protein structure

Primary Structure

Detected by Edman’s degradation technique

Secondary, tertiary and Quaternary structure

Three dimensional structure detected by,

1. X-ray diffraction / Crystallography

2. Nuclear Magnetic resonance

Mass spectrometry is used to access the molecular size. Q

X-ray diffraction crystallography is best method for detection of protein structure. Q

On account of its superior sensitivity, speed, and versatility, mass spectrometry (MS) has replaced the Edman method as the principle method for determining the sequences of peptides and proteins.

Amino Acids, Proteins and Porphyrins Flashcard List

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