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Biochemistry

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Purines, Pyrimidines and Nucleic Acid Metabolism

Question
58 out of 59
 

The rate of DNA synthesis in a culture of cells could be most accurately determined by measuring the incorporation of which of the following radioactive compounds?



A Adenine.
B Guanine.

C Phosphate.
D Thymidine.

Ans. D Thymidine

a. Because thymidine is essentially found only in DNA, its incorporation would most accurately reflect the rate of DNA synthesis. Uridine is found only in RNA and could be used to measure the rate of RNA synthesis.

b. Phosphate, adenine, and guanine are present in both DNA and RNA, and could not be used to specifically measure synthesis of either one.

Purines, Pyrimidines and Nucleic Acid Metabolism Flashcard List

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