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Biochemistry

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Purines, Pyrimidines and Nucleic Acid Metabolism

Question
27 out of 59
 

Two plants grown in garden soil, one to express Growth Fluoro Protein (GFP) and other firefly luciferase. In dark, which of the following will be seen? (AIPG 2010)



A Plant expressing GPF will glow

B Plant expressing firefly luciferase will glow

C Both the plants will glow

D Both the plants will not glow

Ans. B Plant expressing firefly luciferase will glow.

Firefly luciferase is by far the most commonly used bioluminescent reporter. Firefly luciferase is slightly different from GFP, a photoprotein. Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein.


Firefly luciferase

Green fluorescent protein (GFP)

1

Most commonly used bioluminescent reporter

Produces fluoroscence

2

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence .

GPF is a photoprotein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to blue light.

3

Luciferase, a oxidative enzyme required.

Photoproteins (GPF) don’t require any special pigments, enzymes or chemicals to set it off.

Once expressed, it simply needs mineral substrates to emit fluorescence.

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to blue light. Photoproteins (GPF) don’t require any special pigments, enzymes or chemicals to set it off. Once expressed, it simply needs mineral substrates to emit fluorescence. GFP was originally recruited from jellyfish. It has the same purpose as the luciferase enzyme in molecular biology, to mark the expression of select genes. At least in the lab, it GFP also requires to shine blue light (or sometimes UV) for it to emit the green fluorescence. It’s such a standard tool now, in mice, plants, flies, fish studies.

Purines, Pyrimidines and Nucleic Acid Metabolism Flashcard List

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