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Biochemistry

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Amino Acids, Proteins and Porphyrins

Question
28 out of 36
 

Which of the following is not true of denatu-ration of proteins? (AIIMS May 2008)



A Change is isoelectric ph

B Structure is not retained

C Biological activity is preserved

D Increased viscosity

Ans. C Biological activity is preserved

a. The double-stranded DNA molecule exhibits properties of a rigid rod and in solution is a viscous material that loses its viscosity upon denaturation.

b. Raising the temperature increases the rate of both uncatalyzed and enzyme-catalyzed reactions by increasing the kinetic energy and the collision frequency of the reacting molecules.

c. However, heat energy can also increase the kinetic energy of the enzyme to a point that exceeds the energy barrier for disrupting the noncovalent interactions that maintain the enzyme’s three-dimensional structure.

d. The polypeptide chain then begins to unfold, or denature, with an accompanying rapid loss of catalytic activity. The temperature range over which an enzyme maintains a stable, catalytically competent conformation depends upon—and typically moderately exceeds—the normal temperature of the cells in which it resides.

e. Enzymes from humans generally exhibit stability at temperatures up to 45–55 °C. By contrast, enzymes from the thermophilic microorganisms that reside in volcanic hot springs or undersea hydrothermal vents may be stable up to or above 100 °C.

Amino Acids, Proteins and Porphyrins Flashcard List

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