Gram Negative Spiral
Which of the following are the special laboratory conditions needed to recover Campylobacter jejuni?
|A||37°C (98.6°F) aerobic on blood agar plates|
|B||37°C (98.6°F) anaerobic on blood agar plates|
|C||42°C (107.6°F) microaerophilic on Skirrow's medium|
|D||42°C (107.6°F) aerobic on Skirrow's medium|
a. The isolation and identification of Campylobacter jejuni can be achieved using special culture characteristics. Three requirements must be met.
b. First, a selective medium is needed. There are several selective media in widespread use: Skirrow's medium uses vancomycin, polymyxin B, and trimethoprim in 7% lysed blood agar; other selective media contain cefoperazone, other antimicrobials, and inhibitory compounds.
c. The selective media are suitable for isolation of C. jejuni at 42Â°C (107.6Â°F); when incubated at 36-37Â°C (96.8-98.6Â°F), other Campylobacters and bacteria may be isolated.
d. Finally, incubation must be in an atmosphere with reduced oxygen and added carbon dioxide. The colonies appear to be colorless or gray and may be watery and spreading or round and convex.