Gram Negative Coccobacilli
Which of the following needs cholesterol and other lipids for growth?
a. One major limiting factor in cultivation of mycoplasmas is their dependence on a highly complex growing medium.
b. This results from the partial or total inability to synthesize the long-chain fatty acids and cholesterol required for membrane synthesis due to their low genetic IQ.
c. These nutrients are usually supplied in the culture medium by the addition of horse serum.