General Virology and DNA Viruses
Which of the following statements is most accurate regarding infection with parvovirus B19?
|A||Parvovirus B19 replicates in the host cell nucleus utilizing a virally encoded DNA polymerase to create a double- stranded DNA intermediate.|
|B||Parvovirus B19 causes severe anemia because it preferentially infects erythrocyte precursors|
|C||Parvovirus B19 can be diagnosed by detection of viral RNA using PCR or hybridization assays|
|D||Parvovirus B19 can cause hydrops fetalis via transplacental infection of a fetus in a seropositive mother.|
a. Parvovirus B19 binds preferentially to the erythrocyte blood group P antigen on erythroid precursor cells; answers A, B, C are incorrect:
b. after entry the single-stranded viral DNA genome is transported to the cell nucleus where the host DNA polymerase synthesizes the complimentary DNA strand;
c. Parvovirus B19 infection can be diagnosed by direct detection of viral DNA not RNA;
d. the fetus of a seropositive mother is protected from infection by maternal circulating antibodies.
Fig: Pathogenesis of diseases caused by B19 parvovirus. A: In children and adults. (TAC, transient aplastic crisis; PRCA, pure red cell aplasia. ) B: In fetal infections.