Which one of the following sequences is appropriate for testing a patient for antibody against the AIDS virus with the ELISA procedure? (The assay is carried out in a plastic plate with an incubation and a wash step after each addition except the final one.)
|A||Patient's serum/enzyme substrate/HIV antigen/ enzyme-labeled antibody against HIV|
|B|| HIV antigen/patient's serum/enzyme-labeled anti body against human gamma globulin/enzyme substrate|
|C|| Enzyme-labeled antibody against human gamma globulin/patient's serum/HIV antigen/enzyme substrate|
|D||enzyme-labeled antibody against HIV/HIV antigen/ patient's serum/enzyme substrate|
1. Similar to fluorescent assays, ELISA involves the use of enzyme labeled antibodies to detect antibodies/ antigens.
2. The appropriate sequence for testing a patient for antibody against the AIDS virus with the ELISA procedure is HIV antigen/ patient's serum/ enzyme-labeled antibody against human gamma globulin/ enzyme substrate-IndirectELISA