DNA Structure and Function
Part of the process of purifying DNA from isolated chromosomes must involve:
|A||AddingDNase, an enzyme that breaks apart DNA|
|B||Adding proteases, enzymes that break apart proteins|
|C||AddingRNase, an enzyme that breaks apart RNA|
|D||Adding detergents, chemicals that break apart lipids|
Chromosomes contain DNA and proteins. It is necessary to keep the DNA intact during isolation for further experiments, so adding DNase would not be wise. Since chromosomes do not contain RNA or lipids, reagents used to degrade these components are not necessary. However, to purify the DNA, proteins must be removed. Proteases are often used to accomplish this.