A Gardner who pricked her finger while pruning some rose bushes develops a local pustule that progressed to an ulcer. Several nodules then developed along the local lymphatic drainage. The most reliable method to identify the etiologic agent is:
|A||Culture of the organism|
|B||Stain the culture with potassium iodide|
|C||India ink preparation|
|D||Skin test for delayed hypersensitivity|
a. The most reliable method of diagnosing S.
b. schenckii is by culture. Specimens are usually biopsy materials or exudate from granulose or ulcerative lesions and are usually streaked on a selective medium such as Sabouraud agar containing antibacterial antibiotics.
c. Initial incubation is usually 25-30°C, followed by growth at 35°C and thereby confirmation by conversion to the yeast form. Staining procedures are usually nonspecific unless fluorescent antibody.