Sterilization and disinfection
Culture media containing egg or serum is typically sterilized by using ?
|C||Hot air oven|
|D||5 % Cresol|
a. Inspissation or fractional sterilization is used for those high protein containing media which can not be sterilized by autoclaving because they contain an ingredient that breaks down at 121 C. Eg. Egg or serum containing media.
b. It is done by keeping the media at 80-85 C for 1 hr for 3 consecutive days.