General Virology and DNA Viruses
Paul Bunnel Test is used to detect Infectious mononucleosis caused by:
|B||Epstein Barr Virus|
|D||All of the above|
Epstein Barr Virus
a. The heterophile Paul – Bunnel test is positive only in disease caused by the EB Virus.
b. Infectious mononucleosis is an acute self limiting illness usually seen in non immune young adults following primary infection with the EB Virus.
c. The infectious mononucleosis syndrome can follow infection by other agents such as cytomegalovirus and toxoplasmosis or as a reaction to non infectious stimuli but these are not associated with formation of heterophile antibodies
d. Heterophile antibodies agglutinate cells from species different from those in the source serum. The transient heterophile antibodies seen in infectious mononucleosis, also known as Paul-Bunnell antibodies, are IgM antibodies detected by the Paul-Bunnell-Davidsohn test for sheep red cell agglutination. The heterophile antibodies of infectious mononucleosis agglutinate sheep or, for greater sensitivity, horse red cells but not guinea pig kidney cells. This adsorption property differentiates this response from the heterophile response found in patients with serum sickness, rheumatic diseases, and some normal individuals. Titers greater than 1:28 or 1:40 (depending on the dilution system used) after absorption with guinea pig cells are considered positive.