Survival of allografts is increased by choosing donors with few major histocompatibility complex (MHC) mismatches with recipients and by use of immunosuppression in recipients. Which one of the following procedures might be a useful measure of immunosuppression?
|A||Administration of corticosteroids to recipient|
|B||Administration of immunoglobulin to recipient|
|C||Destruction of donor B cells|
|D||Destruction of donor T cells|
a. Allograft rejection is primarily a T cell response to foreign tissue. The corticosteroids reduce inflammatory response and are generally administered by cytotoxic drugs, such as cyclosporine.
b. Lymphoid irradiation is usually done so that the bone marrow is shielded. This removes lymphocytes from lymph nodes and spleen while allowing the patient to have the capacity to regenerate new T and B cells. Likewise, antilymphocyte globulin will destroy the recipient’s lymphocytes, especially T cells.
c. Destruction of donor B cells and T cells would not play a role in the immunosuppression of the graft recipient. In graft crises, monoclonal antibody to CD3 is sometimes given. This targets mature T lymphocytes for destruction.
d. Hybridoma technology by Kohler and Milstein
e. HAT medium contains hypoxanthine, aminopterin, thymidine.
f. Immunotoxin consist of monoclonal antibody and Diphtheria toxin used in lymphoma and leukaemia.
g. Magic bullets are monoclonal antibody + cytotoxic drugs.