The substrate concentration used for determining the activity of an enzyme having Km=x μm will be (LQ)
- Km is Michale is Menten constant. It is numerically equal to the substrate concentration at which the reaction velocity is equal to 1/2 Vmax.
- Now, in the question,
Km = xμm and
Km is the substrate concentration at which reaction velocity is 1/2 Vmax.
So, the maximum activity of the enzyme would be at a substrate concentration of 2xμm.